Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody at 1:1000 at each lane.| Product Name | GOT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate t |
| Product description | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.1) Schiele,F., et.al., Clin. Chem. 35 (6), 926-930 (1989) |
| Immunogen | This GOT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human GOT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOT2 |
|---|---|
| Alternative Names | Aspartate aminotransferase mitochondrial mAspAT Fatty acid-binding protein FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate t |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
