Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.1) Karumanchi,S.A., Mol. Cell(4), 811-822 (2001)
Immunogen
This GPC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human GPC4.
Western blot analysis of GPC4 Antibody in 293 cell line lysates (35ug/lane)
Application
Western blot analysis of GPC4 Antibody in K562 cell line lysates (35ug/lane)
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.