Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody .AP9786b was diluted at 1:1000 at each lane.| Product Name | GPI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, AMF, Neuroleukin, NLK, Phosphoglucose isomerase, PGI, Phosphohexose isomerase, PHI, Sperm antigen 36, SA-36, GPI |
| Product description | GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.1) Shih, W.L., et al. Cancer Lett. 290(2):223-237(2010) |
| Immunogen | This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPI |
|---|---|
| Alternative Names | Glucose-6-phosphate isomerase GPI Autocrine motility factor AMF Neuroleukin NLK Phosphoglucose isomerase PGI Phosphohexose isomerase PHI Sperm antigen 36 SA-36 GPI |
| Molecular Weight(MW) | 63 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody .AP9786b was diluted at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
