Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in IHC on Human Lung.| Product Name | GPNMB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPNMB, HGFIN, NMB |
| Product description | GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential.The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.1) Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPNMB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GPNMB antibody can be used for detection of GPNMB by ELISA at 1:312500. GPNMB antibody can be used for detection of GPNMB by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPNMB |
|---|---|
| Alternative Names | GPNMB HGFIN NMB |
| Molecular Weight(MW) | 60 kDa, 64 kDa |
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in IHC on Human Lung.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GPNMB antibody can be used for detection of GPNMB by ELISA at 1:312500. GPNMB antibody can be used for detection of GPNMB by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GPNMB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
