Application
Immunohistochemistry of GPR3 in human spleen tissue with GPR3 antibody at 2.5 μg/mL.Application
Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of GPR3 in Human Spleen cells with GPR3 antibody at 20 μg/mL.| Product Name | GPR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPR3 Antibody: ACCA, ACCA, G-protein coupled receptor 3, ACCA orphan receptor |
| Product description | GPR3 Antibody: GPR3, also known as ACCA, is a G-protein coupled receptor that constitutively activates adenylate cyclase and is highly expressed in the central nervous system. Overexpression of GPR3 stimulates the production of amyloid-beta peptide (Abeta), the deposition of which is one of the pathological hallmarks of Alzheimer 's disease (AD), while the ablation of GPR3 prevented the accumulation of Abeta in vitro and in an AD mouse model. This is of particular interest because of the proximity of a reported candidate Alzheimer 's disease (AD) locus, suggesting that GPR3 may be a potential therapeutic target for the treatment of AD. GPR3 has also been shown to block the proliferation of cerebellar granule cell precursors (GCP) during postnatal development by inhibiting the Shh-induced proliferation of GCP, indicating that GPR3 activation may represent one of the signals that triggers the postnatal cell cycle exit and terminal differentiation of GPCs.1) Marchesse A, Docherty JM, Nguyen T, et al. Cloning of human genes encoding novel G protein-coupled receptors. Genomics1994; 23:609-18. |
| Immunogen | GPR3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human GPR3. The immunogen is located within the last 50 amino acids of GPR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GPR3 antibody can be used for detection of GPR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR3 |
|---|---|
| Alternative Names | GPR3 Antibody: ACCA ACCA G-protein coupled receptor 3 ACCA orphan receptor |
| Molecular Weight(MW) | Predicted: 36 kDa Observed: 36 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of GPR3 in human spleen tissue with GPR3 antibody at 2.5 μg/mL.
Application
Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of GPR3 in Human Spleen cells with GPR3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | GPR3 antibody can be used for detection of GPR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GPR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GPR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
