Application
Flow cytometric analysis of 293 cells usin(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma wit, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in HL60 cell line lysates (35ug/lane)| Product Name | GPT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1 |
| Product description | GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.1) Yang R.-Z., Blaileanu G.Genomics 79:445-450(2002) |
| Immunogen | This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-112 amino acids from the N-terminal region of human GPT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GPT |
|---|---|
| Alternative Names | Alanine aminotransferase 1 ALT1 Glutamate pyruvate transaminase 1 GPT 1 Glutamic--alanine transaminase 1 Glutamic--pyruvic transaminase 1 GPT AAT1 GPT1 |
| Molecular Weight(MW) | 55 kDa |
Application
Flow cytometric analysis of 293 cells usin(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma wit, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in HL60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
