Application
Immunohistochemistry of GPVI in human heart tissue with GPVI antibody at 10 μg/mL.Application
Immunofluorescence of GPVI in Human Heart tissue with GPVI antibody at 20 μg/mL.Application
Western blot analysis of GPVI in K562 cell lysate with GPVI antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | GPVI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPVI Antibody: GPIV, GPVI, BDPLT11, Platelet glycoprotein VI, Glycoprotein 6 |
| Product description | GPVI Antibody: Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. GPVI deficiency can result in bleeding disorders. Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds.1) Jarvis GE, Atkinson BT, Snell DC, et al. Distinct roles of GPVI and integrin alpha(2) beta(1) in platelet shape change and aggregation induced by different collagens. Br. J. Pharmacol. 2002; 137:107-17. |
| Immunogen | GPVI antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of isoform 2 of the human GPVI. The immunogen is located within the last 50 amino acids of GPVI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GPVI antibody can be used for detection of GPVI by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GP6 |
|---|---|
| Alternative Names | GPVI Antibody: GPIV GPVI BDPLT11 Platelet glycoprotein VI Glycoprotein 6 |
| Molecular Weight(MW) | Predicted: 68 kDa Observed: 67 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | At least three GPVI isoforms are known to exist; this antibody will detect the two longest isoforms. Because of its extensive glycosylation, GPVI often migrates at a higher than expected molecular weight in SDS-PAGE. |
Application
Immunohistochemistry of GPVI in human heart tissue with GPVI antibody at 10 μg/mL.
Application
Immunofluorescence of GPVI in Human Heart tissue with GPVI antibody at 20 μg/mL.
Application
Western blot analysis of GPVI in K562 cell lysate with GPVI antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | GPVI antibody can be used for detection of GPVI by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GPVI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GPVI Antibody is supplied in PBS containing 0.02% sodium azide. |
