Application
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | GR (phospho Ser226) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCCR, GCR, GRL;, NR3C1, |
| Product description | Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.1) Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) J Biol Chem 289, 10235-51 |
| Immunogen | GR (phospho Ser226) antibody was raised against a peptide sequence around phosphorylation site of serine 226 (L-L-S (p)-P-L) derived from Human GR. |
| Modification | p-Ser226 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR3C1 |
|---|---|
| Alternative Names | GCCR GCR GRL NR3C1 |
| Molecular Weight(MW) | 86 kDa |
| Tissue Specificity | The antibody detects endogenous level of GR only when phosphorylated at serine 226. |
| Entrez Gene | 2908 |
|---|---|
| Protein Accession | NP_000167.1 |
Application
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
