Application
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.Application
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | GRB10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.1) Morrione A. Grb10 proteins in insulin-like growth factor and insulin receptor signaling (review). Int. J. Mol. Med. 2000; 5:151-4. |
| Immunogen | GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10. The immunogen is located within amino acids 440 - 490 of GRB10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRB10 |
|---|---|
| Alternative Names | GRB10 Antibody: RSS IRBP MEG1 GRB-IR Grb-10 GRBIR KIAA0207 Growth factor receptor-bound protein 10 GRB10 adapter protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Tissue Specificity | At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms. |
Application
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.
Application
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | GRB10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GRB10 Antibody is supplied in PBS containing 0.02% sodium azide. |
