This gene is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer.This gene is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.1) Sun, J., (2007) Mol. Endocrinol. 21 (11), 2651-2662.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GREB1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GREB1 antibody can be used for detection of GREB1 by ELISA at 1:62500. GREB1 antibody can be used for detection of GREB1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
GREB1 antibody can be used for detection of GREB1 by ELISA at 1:62500. GREB1 antibody can be used for detection of GREB1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GREB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.