Application
Western blot analysis of Grik4 in Rat brain tissue lysate with Grik4 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of Grik4 in Human Brain cells with Grik4 antibody at 20 μg/mL.Application
Immunohistochemical staining of human brain tissue using Grik4 antibody at 2.5 μg/mL.| Product Name | Grik4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Grik4 Antibody: KA1, EAA1, GRIK, GluK4, Glutamate receptor ionotropic, kainate 4, Excitatory amino acid receptor 1 |
| Product description | Grik4 Antibody: Grik4 codes for the KA1 subunit of kainate-type ionotropic glutamate receptors which are critical regulators of network activity that act by modifying neuronal excitability, directly and indirectly, through GABAergic interneurons. Five subunits can assemble to form kainate receptors (KARs): GluR5 (coded by Grik1), GluR6 (coded by Grik2), and GluR7 (coded by Grik3) are the low-affinity subunits, and KA1 and KA2 are the high-affinity subunits. In the adult brain, KARs are located pre- and postsynaptically on pyramidal cells and on interneurons. Kainate receptors on GABA-containing interneurons enhance GABA release and thereby downregulate glutamatergic signaling. KARs have been implicated in numerous psychiatric disorders. Case control studies show significant association of Grik4 with both schizophrenia and bipolar disorder.1) Tanaka K. Functions of glutamate transporters in the brain. Neurosci. Res.2000; 37:15-9. |
| Immunogen | Grik4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of the human Grik4. The immunogen is located within amino acids 30 - 80 of Grik4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Grik4 antibody can be used for detection of Grik4 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, Grik4 often migrates at a lower molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRIK4 |
|---|---|
| Alternative Names | Grik4 Antibody: KA1 EAA1 GRIK GluK4 Glutamate receptor ionotropic kainate 4 Excitatory amino acid receptor 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Grik4 in Rat brain tissue lysate with Grik4 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of Grik4 in Human Brain cells with Grik4 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human brain tissue using Grik4 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | Grik4 antibody can be used for detection of Grik4 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, Grik4 often migrates at a lower molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Grik4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Grik4 Antibody is supplied in PBS containing 0.02% sodium azide. |
