Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-GRK5 Pab in mouse brain tissue lysateApplication
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | GRK5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GRK5, GPRK5 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).1) Pronin, A.N., et al., J. Biol. Chem. 275(34):26515-26522 (2000). |
| Immunogen | This GRK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-590 amino acids from the C-terminal region of human GRK5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GRK5 |
|---|---|
| Alternative Names | G protein-coupled receptor kinase 5 G protein-coupled receptor kinase GRK5 GRK5 GPRK5 |
| Molecular Weight(MW) | 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-GRK5 Pab in mouse brain tissue lysate
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
