GSC regulates chordin (CHRD). GSC may play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis.This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.1) Kohler, A., (2008) Am. J. Med. Genet. A 146 (1), 117-123.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GSC.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISA,WB
GSC antibody can be used for detection of GSC by ELISA at 1:12500. GSC antibody can be used for detection of GSC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in WB on Human 786-0 at 1 ug/ml.
Application
Antibody used in IHC on Human Testis at 5.0 ug/ml.
Application
Antibody used in IHC on Human Tonsil at 5.0 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
GSC antibody can be used for detection of GSC by ELISA at 1:12500. GSC antibody can be used for detection of GSC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GSC antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.