Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21).Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21).| Product Name | GSK3a (phospho Ser21) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPTL, MTBT1, TAU, Factor A, GSK-3 alpha, kinase GSK3-alpha |
| Product description | Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.1) Barry FA, et al. (2003) FEBS Lett. 553 (1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116 (Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141. |
| Immunogen | GSK3a (Phospho-Ser21) antibody was raised against a peptide sequence around phosphorylation site of serine 21 (T-S-S (p) -F-A) derived from Human GSK3α. |
| Modification | p-Ser21 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSK3A |
|---|---|
| Alternative Names | MAPTL MTBT1 TAU Factor A GSK-3 alpha kinase GSK3-alpha |
| Molecular Weight(MW) | 51 kDa |
| Tissue Specificity | This antibody detects endogenous level of GSK3α only when phosphorylated at serine 21and does not detect GSK-3β when phosphorylated at Ser9. |
| Entrez Gene | 2931 |
|---|---|
| Protein Accession | NP_063937.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21).
Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
