Application
Western blot analysis of lysed extracts from HL60, HT29 and 293 cells using GSK3β (Ab-216).| Product Name | GSK3beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta |
| Product description | Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.1) Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137 (1-2): 193-201Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316 (2): 411-415Fan G, et al. J Biol Chem 2003 Dec 26; 278 (52): 52432-52436Liao X, et al. Mol Cancer Ther 2003 Nov; 2 (11): 1215-1222 |
| Immunogen | GSK3beta (Ab-216) antibody was raised against a peptide sequence around aa.214~218 (V-S-Y-I-C) derived from Human GSK3b (GSK3-beta ). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSK3B |
|---|---|
| Alternative Names | Factor A GSK-3 beta Protein kinase GSK-3-beta kinase GSK-3 beta |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | This antibody detects endogenous level of total GSK3β protein. |
| Entrez Gene | 2932 |
|---|---|
| Protein Accession | NP_001139628.1 |
Application
Western blot analysis of lysed extracts from HL60, HT29 and 293 cells using GSK3β (Ab-216).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
