Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Tyr216). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using GSK3β (Phospho-Tyr216).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using GSK3β (Phospho-Tyr216).| Product Name | GSK3beta (phospho Tyr216) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLK1, VEGFR2, Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta |
| Product description | Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.1) Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137 (1-2): 193-201Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316 (2): 411-415Fan G, et al. J Biol Chem 2003 Dec 26; 278 (52): 52432-52436Liao X, et al. Mol Cancer Ther 2003 Nov; 2 (11): 1215-1222 |
| Immunogen | GSK3beta (Phospho-Tyr216) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y (p) -I-C) derived from Human GSK3β. |
| Modification | p-Tyr216 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSK3B |
|---|---|
| Alternative Names | FLK1 VEGFR2 Factor A GSK-3 alpha/beta kinase GSK3-alpha/beta |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | This antibody detects endogenous level of GSK3β only when phosphorylated at tyrosine 216. |
| Entrez Gene | 2932 |
|---|---|
| Protein Accession | NP_001139628.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Tyr216). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using GSK3β (Phospho-Tyr216).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using GSK3β (Phospho-Tyr216).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
