Application
Immunofluorescence of GSTP1 in Human Lung cells with GSTP1 antibody at 20 μg/mL.Application
Immunohistochemical staining of human lung tissue using GSTP1 antibody at 2.5 μg/mL.Application
Western blot analysis of GSTP1 in Jurkat cell lysate with GSTP1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | GSTP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSTP1 Antibody: PI, DFN7, GST3, GSTP, FAEES3, HEL-S-22, Glutathione S-transferase P, GST class-pi |
| Product description | GSTP1 Antibody: Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer. More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson's disease. CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer.1) Pearson WR. Phylogenies of glutathione transferase families. Methods Enzymol.2005; 401:186-204. |
| Immunogen | GSTP1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human GSTP1. The immunogen is located within amino acids 90 - 140 of GSTP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GSTP1 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSTP1 |
|---|---|
| Alternative Names | GSTP1 Antibody: PI DFN7 GST3 GSTP FAEES3 HEL-S-22 Glutathione S-transferase P GST class-pi |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Immunofluorescence of GSTP1 in Human Lung cells with GSTP1 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human lung tissue using GSTP1 antibody at 2.5 μg/mL.
Application
Western blot analysis of GSTP1 in Jurkat cell lysate with GSTP1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | GSTP1 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GSTP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GSTP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
