Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | GTF2A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTF2A1, MGC129969, MGC129970, TF2A1, TFIIA, TFIIAL, TFIIA-42 |
| Product description | GTF2A1 belongs to the TFIIA subunit 1 family. It is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. This protein induces a conformational change within the TBP/TATA complex that enhances its stability under both in vitro and physiological salt conditions.1) Hieb, A.R., (2007) J. Mol. Biol. 372 (3), 619-632. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GTF2A1 antibody can be used for detection of GTF2A1 by ELISA at 1:62500. GTF2A1 antibody can be used for detection of GTF2A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2A1 |
|---|---|
| Alternative Names | GTF2A1 MGC129969 MGC129970 TF2A1 TFIIA TFIIAL TFIIA-42 |
| Molecular Weight(MW) | 41 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GTF2A1 antibody can be used for detection of GTF2A1 by ELISA at 1:62500. GTF2A1 antibody can be used for detection of GTF2A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
