GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.1) Tubon, T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
GTF2B antibody can be used for detection of GTF2B by ELISA at 1:312500. GTF2B antibody can be used for detection of GTF2B by western blot at 4.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
GTF2B antibody can be used for detection of GTF2B by ELISA at 1:312500. GTF2B antibody can be used for detection of GTF2B by western blot at 4.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt