Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | H1FOO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H1FOO, MGC50807, osH1 |
| Product description | H1FOO may play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. H1FOO is essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes.1) Tanaka, M., (2005) Biol. Reprod. 72 (1), 135-142 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human H1FOO. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| H1FOO antibody can be used for detection of H1FOO by ELISA at 1:312500. H1FOO antibody can be used for detection of H1FOO by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1FOO |
|---|---|
| Alternative Names | H1FOO MGC50807 osH1 |
| Molecular Weight(MW) | 36 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | H1FOO antibody can be used for detection of H1FOO by ELISA at 1:312500. H1FOO antibody can be used for detection of H1FOO by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store H1FOO antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
