Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | H2AFY Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2AFY, H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2 |
| Product description | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFY is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Bernstein, E., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (5), 1533-1538. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human H2AFY. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| H2AFY antibody can be used for detection of H2AFY by ELISA at 1:12500. H2AFY antibody can be used for detection of H2AFY by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AFY |
|---|---|
| Alternative Names | H2AFY H2A.y H2A/y H2AF12M H2AFJ MACROH2A1.1 mH2A1 macroH2A1.2 |
| Molecular Weight(MW) | 39 kDa |
| Entrez Gene | 9555 |
|---|---|
| Protein Accession | NP_001035248 |
Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | H2AFY antibody can be used for detection of H2AFY by ELISA at 1:12500. H2AFY antibody can be used for detection of H2AFY by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store H2AFY antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
