Application
Fluorescent confocal image of Hela cell stained with HBO1/MYST2 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HBO1/MYST2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugateApplication
Formalin-fixed and paraffin-embedded human testis tissue reacted with HBO1/MYST2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | HBO1/MYST2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HBO1/MYST2 is a histone acetyltransferase which specifically represses AR-mediated transcription. It may play a role in DNA replication.1) Wu,Z.Q., Proc. Natl. Acad. Sci. U.S.A. 105 (6), 1919-1924 (2008) |
| Immunogen | This HBO1/MYST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-246 amino acids from the Central region of human HBO1/MYST2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | KAT7 |
|---|---|
| Alternative Names | Histone acetyltransferase KAT7 Histone acetyltransferase binding to ORC1 Lysine acetyltransferase 7 MOZ YBF2/SAS3 SAS2 and TIP60 protein 2 MYST-2 KAT7 HBO1 HBOa MYST2 |
| Molecular Weight(MW) | 71 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Fluorescent confocal image of Hela cell stained with HBO1/MYST2 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HBO1/MYST2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugate
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HBO1/MYST2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
