Application
Antibody used in WB on mouse brain at 1:1000 (Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus ).Application
Antibody used in WB on Rat 1 ug/ml.Application
Antibody used in WB on Human Thymus at 1.25 ug/ml.| Product Name | HDAC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.1) Hsu, M.C., et al., (2006) J. Biol. Chem. 281 (8), 4718-4725. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HDAC1 antibody can be used for detection of HDAC1 by ELISA at 1:312500. HDAC1 antibody can be used for detection of HDAC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HDAC1 |
|---|---|
| Alternative Names | HDAC1 HD1 RPD3 GON-10 RPD3L1 |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on mouse brain at 1:1000 (Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus ).
Application
Antibody used in WB on Rat 1 ug/ml.
Application
Antibody used in WB on Human Thymus at 1.25 ug/ml.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | HDAC1 antibody can be used for detection of HDAC1 by ELISA at 1:312500. HDAC1 antibody can be used for detection of HDAC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HDAC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
