Application
Western blot analysis of HDAC1 in human brain tissue lysate with HDAC1 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | HDAC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1,2). HDAC1 gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (3,4).1) Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11. |
| Immunogen | HDAC1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human HDAC1. The immunogen is located within the last 50 amino acids of HDAC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HDAC1 antibody can be used for detection of HDAC1 by Western blot at 1 - 2 μg/ml. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC1 |
|---|---|
| Alternative Names | HDAC1 Antibody: HD1 RPD3 GON-10 RPD3L1 Histone deacetylase 1 HD1 |
| Molecular Weight(MW) | Predicted: 53 kDa Observed: 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | HDAC1 antibody is human, mouse and rat reactive. HDAC1 antibody is predicted to not cross-react with other members of the HDAC family. |
Application
Western blot analysis of HDAC1 in human brain tissue lysate with HDAC1 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | HDAC1 antibody can be used for detection of HDAC1 by Western blot at 1 - 2 μg/ml. |
| Form | Liquid |
|---|---|
| Storage Instructions | HDAC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | HDAC1 antibody is supplied in PBS containing 0.02% sodium azide. |
