Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5 (Phospho-Ser498).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum starvation using HDAC5 (Phospho-Ser498).| Product Name | HDAC5 (phospho Ser498) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.1) Doppler H, et al. (2005) J Biol Chem. 280 (15): 15013-15019. McKinsey TA, et al. (2000) Nature. 408 (6808): 106-111. |
| Immunogen | HDAC5 (Phospho-Ser498) antibody was raised against a peptide sequence around phosphorylation site of serine 498 (T-Q-S (p) -S-P) derived from Human HDAC5/7. |
| Modification | p-Ser498 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC5 |
|---|---|
| Alternative Names | HD5 NY-CO-9 NFKB3 HD5 |
| Molecular Weight(MW) | 124 kDa |
| Tissue Specificity | This antibody detects endogenous level of HDAC5 only when phosphorylated at serine498. |
| Entrez Gene | 10014 |
|---|---|
| Protein Accession | NP_001015053.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5 (Phospho-Ser498).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum starvation using HDAC5 (Phospho-Ser498).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
