Application
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222).Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).| Product Name | HER2 (phospho Tyr1221 / Tyr1222) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.1) Marone R, et al. (2004) Nat Cell Biol; 6 (6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93. |
| Immunogen | HER2 (Phospho-Tyr1221/Tyr1222) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y (p) -Y (p) -W) derived from Human HER2. |
| Modification | p-Tyr1221 / Tyr1222 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERBB2 |
|---|---|
| Alternative Names | NEU NGL HER2 TKR1 CD340 HER-2 MLN19 HER-2/neu GP3A C-erbB-2 ErbB2 |
| Molecular Weight(MW) | 185 kDa |
| Tissue Specificity | This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1221/tyrosine1222. |
| Entrez Gene | 2064 |
|---|---|
| Protein Accession | NP_001005862.1 |
Application
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222).
Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
