Application
Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using HER2 (Phospho-Tyr877).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr877).| Product Name | HER2 (phospho Tyr877) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.1) Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444. Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504. |
| Immunogen | HER2 (Phospho-Tyr877) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y (p) -H-A) derived from Human HER2. |
| Modification | p-Tyr877 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERBB2 |
|---|---|
| Alternative Names | NEU NGL HER2 TKR1 CD340 HER-2 MLN19 HER-2/neu BHLHE39 C-erbB-2 ErbB2 |
| Molecular Weight(MW) | 185 kDa |
| Tissue Specificity | This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877. |
| Entrez Gene | 2064 |
|---|---|
| Protein Accession | NP_001005862.1 |
Application
Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using HER2 (Phospho-Tyr877).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr877).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
