Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3 (Phospho-Tyr1328). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3 (phospho-Tyr1328).Application
Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using Her3/ErbB3 (phospho-Tyr1328).| Product Name | Her3 / ErbB3 (phospho Tyr1328) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Binds and is activated by neuregulins and NTAK.1) Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938. Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301. Kim, H. H. et al. (1994) J. Biol. Chem. 269, 24747-24755. Yarden, Y. and Sliwkowski, M. X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137. |
| Immunogen | Her3/ErbB3 (phospho-Tyr1328) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y (p) -W-H) derived from Human Her3/ErbB3. |
| Modification | p-Tyr1328 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERBB3 |
|---|---|
| Alternative Names | HER3 LCCS2 ErbB-3 c-erbB3 erbB3-S MDA-BF-1 c-erbB-3 p180-ErbB3 p45-sErbB3 p85-sErbB3 KIAA0288 Tyrosine kinase-type cell surface receptor HER3 |
| Molecular Weight(MW) | 185 kDa |
| Tissue Specificity | This antibody detects endogenous level of Her3/ErbB3 only when phosphorylated at tyrosine 1328. |
| Entrez Gene | 2065 |
|---|---|
| Protein Accession | NP_001005915.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3 (Phospho-Tyr1328). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3 (phospho-Tyr1328).
Application
Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using Her3/ErbB3 (phospho-Tyr1328).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
