Application
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.Application
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibodyat 20 μg/mL.Application
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibodyat 5 μg/mL.Application
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.Application
Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.| Product Name | HES5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).1) Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34. |
| Immunogen | HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HES5 |
|---|---|
| Alternative Names | Hairy and enhancer of split 5 hes family bHLH transcription factor 5 bHLHb38 |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 17 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms. |
| Entrez Gene | 388585 |
|---|---|
| Protein Accession | NP_001010926 |
Application
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.
Application
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibodyat 5 μg/mL.
Application
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
Application
Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HES5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | HES5 antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM280858