Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates.1) Elagib, K.E., et al., (2004) Mol. Cell. Biol. 24 (17), 7779-7794.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HEY1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
HEY1 antibody can be used for detection of HEY1 by ELISA at 1:62500. HEY1 antibody can be used for detection of HEY1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Application Notes
HEY1 antibody can be used for detection of HEY1 by ELISA at 1:62500. HEY1 antibody can be used for detection of HEY1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HEY1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt