Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | HIBADH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIBADH, MGC40361, NS5ATP1 |
| Product description | 3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD (+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD (+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD (+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD (+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.1) Hughes, G.J., (1993) Electrophoresis 14 (11), 1216-1222. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIBADH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HIBADH antibody can be used for detection of HIBADH by ELISA at 1:312500. HIBADH antibody can be used for detection of HIBADH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIBADH |
|---|---|
| Alternative Names | HIBADH MGC40361 NS5ATP1 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | HIBADH antibody can be used for detection of HIBADH by ELISA at 1:312500. HIBADH antibody can be used for detection of HIBADH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HIBADH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
