Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of HIF1Alpha Antibody in mouse cerebellum tissue lysates (35ug/lane)Application
Western blot analysis of HIF1Alpha Antibody in MDA-MB231 cell line lysates (35ug/lane)| Product Name | HIF1Alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).1) Favaro,E., Am. J. Pathol. 173 (4), 1186-1201 (2008) Alexandru,G., Cell 134 (5), 804-816 (2008) Linke,S., J. Biol. Chem. 279 (14), 14391-14397 (2004) |
| Immunogen | This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HIF1A |
|---|---|
| Alternative Names | Hypoxia-inducible factor 1-alpha HIF-1-alpha HIF1-alpha ARNT-interacting protein Basic-helix-loop-helix-PAS protein MOP1 Class E basic helix-loop-helix protein 78 bHLHe78 Member of PAS protein 1 PAS domain-containing protein 8 HIF1A BHLHE78 MOP1 |
| Molecular Weight(MW) | 93 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of HIF1Alpha Antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Western blot analysis of HIF1Alpha Antibody in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
