Application
Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
HIF1Alpha Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).Application
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).| Product Name | HIF1Alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.1) Lee, M.N., et al. J. Natl. Cancer Inst. 102(6):426-442(2010) Mayer, A., et al. Adv. Exp. Med. Biol. 662, 399-405 (2010)Brouwer, E., et al. Clin. Exp. Rheumatol. 27(6):945-951(2009) |
| Immunogen | This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:250 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HIF1A |
|---|---|
| Alternative Names | Hypoxia-inducible factor 1-alpha HIF-1-alpha HIF1-alpha ARNT-interacting protein Basic-helix-loop-helix-PAS protein MOP1 Class E basic helix-loop-helix protein 78 bHLHe78 Member of PAS protein 1 PAS domain-containing protein 8 HIF1A BHLHE78 MOP1 |
| Molecular Weight(MW) | 93 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
HIF1Alpha Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).
Application
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).| Application Notes | For WB starting dilution is: 1:250 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
