Application
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 μg/mL.Application
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | HINT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.1) Lima CD, Klein MG, Weinstein IB, et al. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA1996; 93:5357-62. |
| Immunogen | HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1. The immunogen is located within the last 50 amino acids of HINT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HINT1 |
|---|---|
| Alternative Names | HINT1 Antibody: HINT NMAN PKCI-1 PRKCNH1 HINT PKCI1 Histidine triad nucleotide-binding protein 1 Protein kinase C inhibitor 1 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 μg/mL.
Application
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
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| Application Notes | HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
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| Storage Instructions | HINT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HINT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
