Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
HJURP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | HJURP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HJURP is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.1) Sebastiani, P., et al. Science (2010) In press : |
| Immunogen | This HJURP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-588 amino acids from the C-terminal region of human HJURP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HJURP |
|---|---|
| Alternative Names | Holliday junction recognition protein 14-3-3-associated AKT substrate Fetal liver-expressing gene 1 protein Up-regulated in lung cancer 9 HJURP |
| Molecular Weight(MW) | 84 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
HJURP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
