Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.Application
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.Application
Antibody used in WB on Human HEP3B at: 1:1000 (Lane 1: 50 ug HEP3B lysate, Lane 2: 50 ug HEP3B lysate).| Product Name | HK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Paudyal, B., (2008) Cancer Sci. 99 (2), 260-266. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HK2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HK2 antibody can be used for detection of HK2 by ELISA at 1:12500. HK2 antibody can be used for detection of HK2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HK2 |
|---|---|
| Alternative Names | HK2 DKFZp686M1669 HKII HXK2 |
| Molecular Weight(MW) | 102 kDa |
Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.
Application
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.
Application
Antibody used in WB on Human HEP3B at: 1:1000 (Lane 1: 50 ug HEP3B lysate, Lane 2: 50 ug HEP3B lysate).| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | HK2 antibody can be used for detection of HK2 by ELISA at 1:12500. HK2 antibody can be used for detection of HK2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HK2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
