Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | HK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hexokinase-2, Hexokinase type II, HK II, Muscle form hexokinase, HK2 |
| Product description | In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.1) Lehto, M., et al., Diabetologia 38(12):1466-1474 (1995). |
| Immunogen | This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the N-terminal region of human HK2 (Hexokinase II). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HK2 |
|---|---|
| Alternative Names | Hexokinase-2 Hexokinase type II HK II Muscle form hexokinase HK2 |
| Molecular Weight(MW) | 102 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
