Application
HLA-DPB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of HLA-DPB1 using rabbit polyclonal HLA-DPB1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.Application
Western Blot at 1:4000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HLA-DPB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, MHC class II antigen DPB1, HLA-DPB1, HLA-DP1B |
| Product description | HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This HLA-DPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the Central region of human HLA-DPB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-DPB1 |
|---|---|
| Alternative Names | HLA class II histocompatibility antigen DP beta 1 chain HLA class II histocompatibility antigen DP(W4) beta chain MHC class II antigen DPB1 HLA-DPB1 HLA-DP1B |
| Molecular Weight(MW) | 29 kDa |
Application
HLA-DPB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of HLA-DPB1 using rabbit polyclonal HLA-DPB1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.
Application
Western Blot at 1:4000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
