Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.Application
Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HLA-G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-60, HLAG |
| Product description | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.1) Walpole,N.G., et.al., J. Mol. Biol. 397 (2), 467-480 (2010) |
| Immunogen | This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-G |
|---|---|
| Alternative Names | HLA class I histocompatibility antigen alpha chain G HLA G antigen MHC class I antigen G HLA-G HLA-60 HLAG |
| Molecular Weight(MW) | 38 kDa |
Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Application
Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM281056