Hmg20b is the component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. Hmg20b is required for correct progression through G2 phase of the cell cycle and entry into mitosis Hmg20b is also required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.1) Percec, I., et al., (2003) Genetics 164 (4), 1481-1494.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse HMG20B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HMG20B antibody can be used for detection of HMG20B by ELISA at 1:1562500. HMG20B antibody can be used for detection of HMG20B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Bladder at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1410 - Mouse Bladder Tissue Lysate
Application Notes
HMG20B antibody can be used for detection of HMG20B by ELISA at 1:1562500. HMG20B antibody can be used for detection of HMG20B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMG20B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.