Application
Immunohistochemistry of HMGB1 in human lung tissue with HMGB1 antibody at 5 μg/mL.Application
Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 μg/mlApplication
Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 μg/mL.| Product Name | HMGB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1 |
| Product description | The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).1) Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7. |
| Immunogen | HMGB1 antibody was raised against a 19 amino acid peptide near the center of human HMGB1. The immunogen is located within amino acids 70 - 120 of HMGB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HMGB1 antibody can be used for detection of HMGB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGB1 |
|---|---|
| Alternative Names | HMGB1 Antibody: HMG1 HMG3 SBP-1 HMG1 High mobility group protein B1 High mobility group protein 1 HMG-1 |
| Molecular Weight(MW) | Predicted: 24 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | HMGB1 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of HMGB1 in human lung tissue with HMGB1 antibody at 5 μg/mL.
Application
Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 μg/ml
Application
Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | HMGB1 antibody can be used for detection of HMGB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | HMGB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
