Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on Human Lung lysate.Application
Antibody used in IHC on Human Liver cell lysates.| Product Name | HMGB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMGB2, HMG2 |
| Product description | This protein is a member of the non-histone chromosomal high mobility group family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in the DNA end-joining processes of DNA double-strand breaks repair and V (D)J recombination.This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V (D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) McCauley, M.J., (2007) J. Mol. Biol. 374 (4), 993-1004. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HMGB2 antibody can be used for detection of HMGB2 by ELISA at 1:312500. HMGB2 antibody can be used for detection of HMGB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGB2 |
|---|---|
| Alternative Names | HMGB2 HMG2 |
| Molecular Weight(MW) | 24 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Lung lysate.
Application
Antibody used in IHC on Human Liver cell lysates.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | HMGB2 antibody can be used for detection of HMGB2 by ELISA at 1:312500. HMGB2 antibody can be used for detection of HMGB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMGB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
