Application
Western blot analysis of lysates from Jurkat, PC-3 cell line (from left to right), using HMGB4 Antibody at 1:1000 at each lane.| Product Name | HMGB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | High mobility group protein B4, HMGB4 |
| Product description | High Mobility Group (HMG) proteins, a group of chromosomal proteins common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation, helping with transcription, replication, recombination, and DNA repair. HMGB4 is HMG2-like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, and is found in a variety of eukaryotic chromosomal proteins. HMGB proteins are phosphorylated to various extents. The existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions. |
| Immunogen | This HMGB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-73 amino acids from the Central region of human HMGB4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGB4 |
|---|---|
| Alternative Names | High mobility group protein B4 HMGB4 |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysates from Jurkat, PC-3 cell line (from left to right), using HMGB4 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
