Application
Western blot analysis in WiDr cell line lysates (35ug/lane).| Product Name | HMGCL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hydroxymethylglutaryl-CoA lyase, mitochondrial, HL, HMG-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HMGCL |
| Product description | HMGCL belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Fu, Z., et al. J. Biol. Chem. 285(34):26341-26349(2010) |
| Immunogen | This HMGCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the Central region of human HMGCL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGCL |
|---|---|
| Alternative Names | Hydroxymethylglutaryl-CoA lyase mitochondrial HL HMG-CoA lyase 3-hydroxy-3-methylglutarate-CoA lyase HMGCL |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
