This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.1) Olsen, J.V., (2006) Cell 127 (3), 635-648.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGCS1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HMGCS1 antibody can be used for detection of HMGCS1 by ELISA at 1:312500. HMGCS1 antibody can be used for detection of HMGCS1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.
Application
Antibody used in WB on Human 293T at 1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
HMGCS1 antibody can be used for detection of HMGCS1 by ELISA at 1:312500. HMGCS1 antibody can be used for detection of HMGCS1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMGCS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.