Application
Flow cytometric analysis of hela cells using HMGCS1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).| Product Name | HMGCS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS |
| Product description | HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.1) Vock,C., Cell. Physiol. Biochem. 22 (5-6), 515-524 (2008) |
| Immunogen | This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | HMGCS1 |
|---|---|
| Alternative Names | Hydroxymethylglutaryl-CoA synthase cytoplasmic HMG-CoA synthase 3-hydroxy-3-methylglutaryl coenzyme A synthase HMGCS1 HMGCS |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Flow cytometric analysis of hela cells using HMGCS1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
