Application
Confocal immunofluorescent analysis of HNF1A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)| Product Name | HNF1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, LFB1, Transcription factor 1, TCF-1, HNF1A, TCF1 |
| Product description | HNF1A is a transcription factor required for the expression of several liver-specific genes. This protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.1) Bach,I., et.al., Genomics(1), 155-164 (1990) |
| Immunogen | This HNF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of human HNF1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNF1A |
|---|---|
| Alternative Names | Hepatocyte nuclear factor 1-alpha HNF-1-alpha HNF-1A Liver-specific transcription factor LF-B1 LFB1 Transcription factor 1 TCF-1 HNF1A TCF1 |
| Molecular Weight(MW) | 67 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Confocal immunofluorescent analysis of HNF1A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
