Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Fetal Liver at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | HNF4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNF4A, TCF, HNF4, MODY, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha |
| Product description | The protein encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.1) Weedon, M.N., et al., (2004) Diabetes 53 (11), 3002-3006. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNF4A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| HNF4A antibody can be used for detection of HNF4A by ELISA at 1:62500. HNF4A antibody can be used for detection of HNF4A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNF4A |
|---|---|
| Alternative Names | HNF4A TCF HNF4 MODY MODY1 NR2A1 TCF14 HNF4a7 HNF4a8 HNF4a9 NR2A21 HNF4alpha |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Fetal Liver at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | HNF4A antibody can be used for detection of HNF4A by ELISA at 1:62500. HNF4A antibody can be used for detection of HNF4A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNF4A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
