Application
Confocal immunofluorescent analysis of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Product Name | HNF4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14 |
| Product description | HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.1) Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010) |
| Immunogen | This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human HNF4A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HNF4A |
|---|---|
| Alternative Names | Hepatocyte nuclear factor 4-alpha HNF-4-alpha Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 TCF-14 Transcription factor HNF-4 HNF4A HNF4 NR2A1 TCF14 |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
