HNF4 was first identified as a DNA binding activity in rat liver nuclear extracts and then was found to be an orphan member of the nuclear receptor superfamily. Binding sites for this factor were identified in many tissue-specifically expressed genes, and the protein was found to be essential for early embryonic development in the mouse1) Ozeki, T., et al., (2003) Pharmacogenetics 13 (1), 49-53.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNF4G.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HNF4G antibody can be used for detection of HNF4G by ELISA at 1:62500. HNF4G antibody can be used for detection of HNF4G by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
HNF4G antibody can be used for detection of HNF4G by ELISA at 1:62500. HNF4G antibody can be used for detection of HNF4G by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNF4G antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.